A novel Ca2+ indicator for long-term tracking of intracellular calcium flux

A novel Ca2+ indicator for long-term tracking of intracellular calcium flux

Blog Article

The major drawback of using Fluo-4 AM is that it requires an organic anion transporter inhibitor, such as probenecid, to prevent leakage.This can hinder the studies that require extended monitoring time and longer cellular retention.To address the issue, a novel Ca2+ indicator, Calbryte 520 AM, was developed.

We compared the performance of Fluo-4 AM and Calbryte 520 100% PURE SHEA BUTTER AM following prolonged incubation periods after cell loading.Cells loaded with Pendant Lighting Calbryte 520 AM retained the dye for up to 24 h while exhibiting significant fluorescence brightness and superior Fmax/F0 ratios (Fmax: fluorescence intensity upon stimulation; F0: intensity before stimulation).It demonstrated that the longer retention of Calbryte 520 AM can be exploited to accommodate for the extended time required when monitoring calcium dynamics.